By A H Rebar; et al
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Additional info for A Guide to Hematology in Dogs and Cats
Increased globulin production is most commonly associated with inflammation; an inflammatory leukogram is generally present. ® Evaluation of serum protein and serum albumin levels are useful in further clarifying the interpretation of plasma protein abnormalities. Hemoglobin Concentration ® Hand held hemoglobinometers provide a simple and rapid method to estimate hemoglobin concentration if automated equipment is not available. 18 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 19 3 LABORATORY METHODS IN HEMATOLOGY ® Some in-clinic chemistry analyzers measure hemoglobin in whole blood samples electrochemically.
The fluidity of normal red cells allow them to traverse tortuous capillary beds leading to close approximation of red cells with tissue cells. This in turn makes gaseous exchange efficient. Physiology Red cell physiology is geared to facilitate function and protect red cell integrity. The primary red cell metabolic pathway is anaerobic glycolysis. The glycolytic pathway allows the cell to produce energy to maintain membrane stability with minimal utilization of oxygen. The red cell also has metabolic pathways (hexose monophosphate shunt and methemoglobin reductase) which protect hemoglobin from oxidation.
When a particle passes through the opening, it causes a change in electrical impedance and a measurable voltage pulse. – The magnitude of the voltage change is proportional to the size of the cell. – Voltage pulses are detected, analyzed and counted electronically. ® The red cell count actually includes both red and white cells, but because the proportion of white cells is usually small, the effect is insignificant unless the animal is simultaneously anemic and leukemic. ® Red cells are lysed in order to obtain a white cell count.