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By Andor Szentivanyi, Herman Friedman, Günther Gillissen

This ebook includes 32 chapters in keeping with the corresponding papers introduced on the overseas Symposium on Antimicrobial brokers and Immunity, held in Siena, Italy on could 2-4, 1985 as a satellite tv for pc Symposium of the 3rd overseas convention on Immunopharmacology, held in Florence, Italy on may possibly 6-10, 1985. As editors we show our profound appreciation and gratitude to the authors who've contributed so richly to this quantity, and we expect that it will possibly no longer be an excessive amount of to wish new cadre of investigators and scholars will proportion this gratitude for those documents of expertise and perception into antibiotic and host-parasite interactions. We owe a truly specified gratitude to Mrs. Christine Abarca for her extraordinary editorial information within the practise of this e-book. Andor Szentivanyi, M.D. college individual Professor Departments of Pharmacology and inner medication collage of South Florida university of drugs Tampa, Florida Herman Friedman, Ph.D. Professor and Chairman division of scientific Microbiology and Immunology college of South Florida university of drugs Tampa, Florida Gunther Gillissen, M.D., Ph.D. Professor and Chairman division of scientific Microbiology school of drugs RWTH, Aachen, F.R.G.

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F. Michael, Activation of complement by cell surface components of Staphylococcus ~, Infect. Immun. 20:388 (1978). B. J. Wilkinson, Y. Kim and P. K. Peterson, Factors affecting complement activation by Staphylococcus aureus cell walls, their components, and mutants altered in teichoic acid, Infect. Immun. 32:216 (1981). 49 IN VITRO EFFECTS OF SUB-MIC'S OF ANTIBIOTICS ON THE ANTIGENICITY AND IMMUNOGENICITY OF PSEUDOMONAS AERUGINOSA Philippe H. Lagrange, Herve Richet and Marie Christine Escande Laboratoire Central de Microbiologie Paris, France INTRODUCTION In addition to the studies on direct or indirect effects of antibiotics (AB) on the host immune responses of normal or infected hosts, some researchers have recently directed their activity toward the pathogenic bacteria themselves (1).

40 In vitro colonization of rabbit bone by Bacteroides intermedius at 48 hr. 00039 ~g/ml) of clindamycin. large aggreates of bacilli adhered to one another and to bone surface. Condensed strands of glycocalyx (arrows GC) were evident. 00097 ~g/ml) of c1indamycin significantly decreased colonization of bacilli to bone. Condensed strands of glycocalyx (arrow GC) attached to bacilli were still visible. Bar = 5 ~m. the bacterial cells. Bacilli, coccobacilli, and coccoid forms--all typical of B. intermedius cell morphology--were present.

2d). The filaments tend to lyse at both ends. Apparent reduced numbers of ribosomes were not observed in the carbenicillin treated bacteria compared to normal (Figure 2d). The rever§ibility of these morphological alterations was observed when Ps. aeruginosa. exposed to 1/2 - 1/3 MIC of carbenicillin. transferred to drug-free agar and incubated 4 hours at 37°C. showed a significant increase in the number of CFU and returned to rod-like morphology. The same findings were also observed when such filamentation in vitro AB treated 53 • ' .

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